Abstract: OBJECTIVE: To discuss mechanism of miRNA-128-1 expression in HEK293 cells and lay the foundation for further study of miRNA-128-1 regulation in glioma. METHODS：Through analyzing UCSC，we found the probable miRNA-128-1 promoter area. We used UCSC and CBIL to obtain the predicted transcription factors binding on the miRNA-128-1 promoter area. Related plasmids were constructed，including pB-miRNA-128-1 promoter，pShNF1，pShc-Myc，pShTAF1，pShYY1，pShMAF，pShRELA2，pShRNUX1 and pShNFkappaB. Cotransfecting pB-miRNA-128-1 promoter and pShRNA into HEK293 cells，we analyzed luciferase activity to identify probable transcription factors which could regulate miRNA-128-1 promoter activity. RESULTS：pShc-Myc suppressed the activity of pB-miRNA-128-1 promoter. pShNFkappaB increased the activity of pB-miRNA-128-1 promoter dramatically. pShNF1，pShTAF1，pShYY1，pShMAF，pShRELA2 or pShRNUX1 showed no obvious influence on the expression of pB-miRNA-128-1. CONCLUSION：In HEK 293 cells，c-Myc upregulated whilst NFkappaB downregulated the promoter activity of miRNA-128-1.

Key words: miRNA-128-1, regulation of expression, HEK293 cells